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. Author manuscript; available in PMC: 2010 Dec 22.
Published in final edited form as: Biochemistry. 2009 Dec 22;48(50):11902–11911. doi: 10.1021/bi901600f

Figure 1.

Figure 1

Effects of cyclooxygenase inhibitors on tyrosyl radical EPR spectrum when added to PGHS-2 before reaction with peroxide. Aliquots of hPGHS-2 holoenzyme (11 μM in 0.1 M KPi, pH 7.2 containing 11 μM phenol, 5% glycerol and 0.1% Tween 20) were incubated with 1.5 eq of the indicated inhibitor on ice for at least 30 min before reaction with 10 eq of EtOOH for 6-8 s. The mixtures were frozen in a dry ice/ethanol bath and their EPR spectra were acquired at 120 K. The EPR signals were normalized to give the same amplitude for all samples. The peak-trough widths indicated in parentheses were determined from the zero-crossings of the first derivative of the EPR signals.