. Author manuscript; available in PMC: 2010 Dec 22.

Published in final edited form as: Biochemistry. 2009 Dec 22;48(50):11902–11911. doi: 10.1021/bi901600f

Table 2.

Inhibitor effects on tyrosyl radical EPR characteristics.

Inhibitor	Linewidth (G)	Linewidth (G)	Relative Half-life^c	Relative Initial Yield^d	Relative Initial Yield^d
	I first^a	ROOH first^b	ROOH first^b	I first^e	ROOH first^b
None	-	28.6 ± 0.4 (n=8)	1.0	1.0	1.0
Nimesulide	25.2 ± 0.5 (n=4)	27.3 ± 0.4 (n=11)	170	0.12	1.4
Flurbiprofen	28.6 ± 0.2 (n=3)	29.0 ± 0.3 (n=9)	12	0.29	1.1
Diclofenac	25.0 ± 0.5 (n=4)	28.3 ± 0.7 (n=5)	0.20	0.17	0.8

Preincubated with 2 eq of inhibitor before reaction with 10 eq of EtOOH for 6-8 s.

Reacted with 2 eq EtOOH for 6-8 s before addition of 20 eq of inhibitor (if any).

Normalized to the tyrosyl radical half-life in the control reacted with only EtOOH. Values are the averages of duplicate determinations.

Normalized to the initial tyrosyl radical intensity in the control reacted with only EtOOH. Values are the averages of duplicate determinations.

Preincubated with 2 eq of inhibitor before reaction with 2 eq of EtOOH for 6-8 s.