Figure 1. P66 ^Shc controls intracellular ROS metabolism.

at multiple sites. P66^Shc (in blue) stimulates ROS production by plasma membrane oxidases through the association with membrane receptor and Rac activation of phagocitic oxidases. Upon phosphorylation and consequent Pin-1-mediated conformational changes, p66^Shc (in red) translocates, through the TIM/TOM mitochondrial import machinery, within the mitochondrial inter-membrane space where it oxidizes reduced cytochrome c and catalyzes the partial reduction of O₂ to H₂O₂. Then, p66^Shc decreases the expression of ROS scavenging enzymes.