Figure 2. Regulatory circuit of p66 ^Shc-mediated fat development.

The scheme recapitulates the pathway of p66^Shc that drives mitochondrial H₂O₂ and its relationship with insulin receptor signaling leading to fat accumulation. Food intake determines energetic substrate availability and insulin stimulates intracellular transduction pathways that regulate gene transcription in order to favor triglyceride accumulation. P66^Shc-mediated ROS production is directly boosted by insulin and in turn potentiates insulin receptor signaling, suppresses the expression of uncoupling proteins and beta oxidation enzymes leading to triglyceride accumulation.