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Apoptotic markers in old mother cells (fraction V) of the mutant afo1Δ strain. (A) phase contrast; (B) same cell as in A stained with Calcofluor White M2R; (C) the same cell stained with DHE indicating a high level of ROS; (D) an old mother cell stained with FITC-annexin V revealing inversion of the plasma membrane; (E) the same cell as in (D) shows absence of staining with propidium iodide revealing intact plasma membrane; (F) TUNEL staining of old afo1Δ cells.
