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Old and young cells of the same strains as in A were isolated by elutriation centrifugation and ERCs were analyzed by gel electrophoresis and Southern blotting with an rDNA-specific probe as described in [19]. Thick arrow: chromosomal rDNA repeats; Thin arrow: ERCs (minicircles). Taken together, the results presented in this figure indicate that longevity in the afo1Δ strain is not influenced by the fob1-deletion or the presence of ERCs.
