. Author manuscript; available in PMC: 2010 Oct 1.

Published in final edited form as: Dev Dyn. 2009 Oct;238(10):2599–2613. doi: 10.1002/dvdy.22092

Table 1. Quantitative analysis of the effects of loss of Prx on the size, proliferation, apoptosis and osteogenesis of the mandibular processes.

Determination of the size, quantification of H3-p positive cells and TUNEL positive cells, as well as the analysis of osteogenesis were performed as described in Materials and Methods. The areas for quantitative analysis included the entire area of the mandibular mesenchyme and the mesenchyme of the medial region. All parameters were measured using ImageJ and values represent means ± S.E. Asterisk indicates statistically significant difference (p< 0.05).

	Stage of development	Prx1+/+; Prx2-/- (control)	Prx1-/-; Prx2-/-
Size (mm²)	E10.5 n=4	0.75 ± 0.01	0.44 ± 0.02*
Size (mm²)	E13.5 n=3	2.62 ± 0.04	1.71 ± 0.09*
TUNEL positive cells (cells/mm²)	E10.5 n=4	59 ± 21	290 ± 145*
TUNEL positive cells (cells/mm²)	E11.5 n=4	30 ± 18	76 ± 32*
H3-P positive cells (cells/mm²)	E10.5 (whole mandible) n=4	444 ± 105	206 ± 85*
H3-P positive cells (cells/mm²)	E10.5 (medial region) n=4	297 ± 97	101 ± 62*
Osteogenesis (mm²)	Runx2 expression n=3	0.36 ± 0.01	0.47 ± 0.02*
Osteogenesis (mm²)	Mason Tricrome n=3	0.06 ± 0.004	0.12 ± 0.01*