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. 2009 Sep 9;26(2):1372–1379. doi: 10.1021/la902443e

Figure 3.

Figure 3

Curtains of double-tethered DNA. Panel A shows a typical example of a single-tethered curtain, in the absence (top) and presence (bottom) of buffer flow. Panels B and C show examples of double-tethered DNA curtains in the absence of any buffer flow, illustrating that the DNA molecules remain extended even in the absence of continual hydrodynamic force. The Cr barrier patterns in panel B were made by ebeam lithography, and those in panel C were made by nanoimprint lithography. Panel D shows the relative anchoring efficiency of the second DNA end as a function of the separation distance between the linear barrier and the polygon array, revealing a peak tethering efficiency corresponding to 13 μm, which corresponds to ∼80% extension of the λ DNA relative to its full contour length (48502 bp, ∼16.5 μm). Panel D shows the percent of digoxigenin-labeled DNA that remains anchored after defined time intervals, revealing a half-life of 69 min.