FIG. 5.

Presence of calcified deposits in the matrix and gene expression in the presence of the growth medium. Graphs (A) and (B) represent fold change in expression profiles of different genes in the mesenchymal and epithelial cells, respectively, after 1, 5, and 10 days in coculture with respect to their expression in monoculture. (C) and (C1) are histological images of von Kossa–stained sections after 8 days of coculture in the growth medium. (D) and (D1) are images of von Kossa–stained sections after 16 days of coculture in the growth medium. (E) and (E1) are images of von Kossa–stained sections after 24 days of coculture in the growth medium. Arrows point to calcium deposition in and around the cells, and in the matrix. (C1), (D1), and (E1) are images of the mesenchymal areas alone. Scale bar represents 20 μm for all images. Color images available online at www.liebertonline.com/ten.