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Western blot analyses for BAX (A) and Bcl-2 (B) in liver cytosol extracts of young and mature vehicle-treated rats. Fifty micrograms of protein were loaded for each lane. Equal loading was confirmed by β-actin immunoblotting. Image analysis of expression of BAX (C) and Bcl-2 (D) was determined by densitometry. Fold increase was calculated vs. respective sham value (time 0) set to 1.0. Results are representative of 3 separate time-course experiments. *P < 0.05 vs. young rats.
