Fig. 3.

A: quantitative assessment of myofibroblast accumulation by immunoreactive interstitial α-smooth muscle actin (α-SMA) in UUO rats. Animals were treated as described in Fig. 1 legend and the expression of interstitial α-SMA was determined. L (gray bars) indicates left, obstructed kidney; R (open bars) indicates right, nonobstructed kidney; n = 6 each. *P < 0.05 vs. control. **P < 0.05 vs. imatinib, 50 mg/kg. B–D: matrix gene expression in UUO rats. mRNA levels encoding Col3A1 (B), Col1A2 (C), and fibronectin (D) in the left (obstructed) kidney (gray bars) and the right (nonobstructed) kidney (open bars); n = 6 each. *P < 0.05 vs. control. **P < 0.05 vs. 50 mg/kg imatinib or 1.5 mg/kg rapamycin.