Fig. 11.

Colocalization of TSC and AQP2 with the increased expression of Na⁺-K⁺-ATPase α₁ in Cln7^−/− kidneys. Kidney frozen sections from 6-day-old Cln7^−/− mice were double immunostained by monoclonal anti-Na⁺-K⁺-ATPase α₁ with polyclonal anti-TSC (distal convoluted tubule marker) (a and b), with anti-AQP2 (collecting duct marker) (d and e), or with anti-claudin-2 (proximal convoluted tubule marker) (g and h) antibodies. Images of c, f, and i were merged images of a and b, d and e, and g and h, respectively. Arrows in a–c, d–f, or g–i indicate the same tubules, respectively. Arrowheads in g–i pointed to the claudin-2 positive tubules that were not colocalized with the tubules with enhanced Na⁺-K⁺-ATPase α₁ signal. Scale bar: 20 μm.