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Salt wasting in Cln7−/− mice. A: urine was directly collected from the bladder of 3-day-old Cln7+/+ (WT) and Cln7−/− (KO) mice. Urine K+, Cl−, Na+, Mg2+, and Ca2+ were measured and normalized to the urine creatinine concentrations (mM ion/mg creatinine). B: the same measurements were conducted in 7-day-old Cln7+/+ and Cln7−/− mice. Each data point represents at least 6 samples and is presented as mean ± SE. *Significantly different from the control (P < 0.05).
