Fig. 9.

Expression of claudin proteins in Cln7^+/+ and Cln7^−/− kidneys. A: kidneys from 1-wk-old Cln7^+/+ and Cln7^−/− mice were removed from the body and frozen in liquid nitrogen. Frozen sections (5 μm) were immunostained with anti-claudin-2, -3, -4, and -8 antibodies and detected by Cy3-conjugated anti-rabbit secondary antibody. Scale bar: 60 μm. B: kidneys from 1-wk-old Cln7^+/+ and Cln7^−/− mice were homogenized and lysed in lysis buffer. A total of 40 μg protein for each sample were loaded on the SDS-polyacrylamide gel. Membranes were blotted against claudin-2, -3, -4, and -8 antibodies. Three independent experiments were repeated.