Table 1.

Real-time RT-PCR primers and probes

Gene	Type	Sequence (5′-3′)
Aldosterone synthase	Forward	AGA AGC CAG CAA CTT TGC TC
	Reverse	GCA GGG CAT GGA TAA ACT TC
	Probe	f CAT GAC CTG AGC CCT GGC AGC C q
SGK1	Forward	GCT TAT GAA CGC TAA CCC CT
	Reverse	GAA AGT CGG AGG GTT TGG C
	Probe	f TCA ACC TGG GTC CGT CCT CCA ACC q
Renin	Forward	ACA GTA TCC CAA CAG GAG AGA CAA G
	Reverse	GCA CCC AGG ACC CAG ACA
	Probe	f TGG CTC TCC ATG CCA TGG ACA TCC q
ENaCα	Forward	AGC GCG TCT TCC AGT GTA C
	Reverse	GAT TTG TTC TGG TTG CAC AGT
	Probe	f CAA CAA TCC CCA AGT GGA CAG GAA GG q
ROMK	Forward	GCA CAG TAG AAT CCA CCA GT
	Reverse	AAA GCA CCT CTT CTG GGA TG
	Probe	f CAA CCT GCC AAG TCC GCA CAT CA q
NKCC2	Forward	AGG CTC TGT CCT ATG TGA GT
	Reverse	CAT GGG TCC GCC TGT TAA G
	Probe	f TAG ACA ACG CTC TGG AAT TAA CCA CAG q
AQP2	Forward	TAC GTG GCT GCC CAG CTG
	Reverse	GGC TGT TGC ATT GTT GTG GA
	Probe	f CAT GAG ATT ACC CCT GTA GAA ATC CGC q
β-Actin	Forward	CTG CCT GAC GGC CAG GTC
	Reverse	CAA GAA GGA AGG CTG GAA AAG A
	Probe	t CAC TAT TGG CAA CGA GCG GTT CCG q

SGK1, serum-glucocorticoid-induced kinase 1; ENaCα, epithelial Na⁺ channel; ROMK, renal outer medullary K⁺ channel; NKCC2, Na⁺-K⁺-2Cl⁻ cotransporter; AQP2, aquaporin-2; f, reporter dye 1 (6-carboxyfluorescein); q, quencher dye (6-carboxytetramethyl1-rhodamine); t, reporter dye2 (tetrachloro-6-carboxyfluorescein).