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. 2009 Oct 30;298(1):H127–H135. doi: 10.1152/ajpheart.00732.2009

Fig. 6.

Fig. 6.

Interstitial flow induces AP-1 DNA binding activity via ERK1/2 activation. ERK1/2 inhibitor PD-98059 abolished flow-induced augmentation of AP-1 binding activity, whereas SB-203580 slightly enhanced flow-induced augmentation of AP-1 binding activity. After 24 h of cell spreading in collagen I gels, media was replaced with either fresh media or 5 μM SB-203580 or 10 μM PD-98059 for 1 h, and the gels were then exposed to 1 cmH2O of interstitial flow either with or without inhibitors for 3 h. After flow, cells were released from collagen gels by incubating with 0.2% collagenase I for 40 min. Nuclear proteins were then extracted followed by AP-1 DNA binding activity assay. Data are presented as means ± SE; n = 3. *P < 0.05 vs. Ctrl; #P < 0.02 vs. flow.