Table 1.

Intracellular concentrations of GSH and GSSG in siRNA-transfected endothelial cells

siRNA	Control	GR	TrxR1	TrxR2
[GSH]	124.1±4.7	136.1±3.3	152.9±9.3*	125.2±2.5
[GSSG]	0.58±0.1	1.13±0.1*	1.44±0.2*	1.13±0.1*

Each data point represents the mean ± SE in nmol/mg protein, derived from four independent experiments, each performed in duplicate. Shown are the concentrations of GSH and GSSG in endothelial cells transfected with control, glutathione reductase (GR), cytosolic Trx reductase (TrxR1), or mitochondrial Trx reductase (TrxR2) small interfering RNA (siRNA). Forty-eight hours following siRNA transfections, the intracellular concentrations of GSH and GSSG were assessed, as detailed in the text.

^*P < 0.01 vs. control siRNA-transfected cells (ANOVA).