Fig. 4.

1-Oleoyl-2-acetyl-sn-glycerol (OAG)-induced [receptor-operated channel (ROC)-mediated] Ba²⁺ entry and TRPC3/6 protein expression in freshly dissociated ASMCs from NT and OH rats. A: representative records showing the time course of the fura-2 fluorescence ratio [fluorescence at 340-to-380-nm excitation (F₃₄₀/F₃₈₀)] signals induced by 80 μM OAG in freshly dissociated ASMCs from NT and OH rats. Extracellular Ca²⁺ was replaced by 1 mM Ba²⁺ during the period indicated on the graph. Nifedipine (10 μM) was applied 10 min before the trace shown and was maintained throughout the experiment. B: summarized data showing the OAG-induced, ROC-mediated Ba²⁺ entry [ROC-mediated Ca²⁺ entry (ROCE)] in 78 NT and 91 OH rat mesenteric ASMCs. Each bar corresponds to data from 8 rats. C–F: Western blot analysis of TRPC6 and TRPC3 expression in ASMCs from NT and OH rats. C and E: representative immunoblots (15 μg protein/lane). Summarized data from 9 (D) and 10 (F) immunoblots (total of 18 rats) were normalized to β-actin. ***P < 0.001 vs. NT ASMCs.