Fig. 6.

OAG-induced (ROC-mediated) Ba²⁺ entry and TRPC6 protein expression in cultured ouabain-treated ASMCs. A and B: representative records showing the time course of the fura-2 fluorescence ratio (F₃₄₀/F₃₈₀) signal induced by 80 μM OAG in control myocytes and ASMCs treated with 10 (A) or 100 nM (B) ouabain. Extracellular Ca²⁺ was replaced by 1 mM Ba²⁺ during the period indicated on the graph. Nifedipine (10 μM) was applied 10 min before the trace shown and was maintained throughout the experiment. C: summarized data showing the OAG-induced, ROC-mediated Ba²⁺ entry (ROCE) in control myocytes (n = 124) and in ASMCs treated with 10 (n = 58) or 100 nM (n = 80) ouabain for 72 h. D: Western blot analysis of TRPC6 expression in control (untreated) myocytes and ASMCs treated with 100 nM ouabain for 48 or 72 h. E: summarized data from 19 immunoblots were normalized to the amount of β-actin. *P < 0.05 and ***P < 0.001 vs. control.