Fig. 7.

Effects of NCX1/silencer (si)RNA and ouabain on NCX1 and TRPC6 expression and OAG-induced (ROC-mediated) Ba²⁺ entry in cultured ASMCs. A–D: Western blot analysis of NCX1 (A and B) and TRPC6 (C and D) expression in control (untreated) ASMCs and cells treated with 100 nM ouabain for 72 h under control conditions and after 77 h of treatment with NCX1/siRNA. B and D: summarized data from 9 (B) and 14 (D) immunoblots. E: representative records showing the time course of the fura-2 fluorescence ratio (F₃₄₀/F₃₈₀) signal induced by 80 μM OAG in a control ASMC and a cell treated with NCX1/siRNA. Extracellular Ca²⁺ was replaced by 1 mM Ba²⁺ during the period indicated on the graph. Nifedipine (10 μM) was applied 10 min before the trace shown and was maintained throughout the experiment. F: summarized data showing the OAG-induced, ROC-mediated Ba²⁺ entry (ROCE) in 42 control myocytes and in 38 cells treated with NCX1/siRNA. *P < 0.05 and ***P < 0.001 vs. control (untreated) cells.