Fig. 4.

p90RSK is involved in the regulation of the brain natriuretic peptide (BNP) promoter. NVMs were transfected with −1818hBNP luciferase and, 3 days later, also infected with an adenovirus encoding either LacZ or dnp90RSK. Cells were treated with PGE₂ for the final 24 h of the experiment and then lysed for the assay of luciferase activity. Luciferase activity in the control cells was arbitrarily assigned a value of 1, and activity in PGE₂-stimulated cells is expressed as the fold increase versus the control. Data are mean ± SE from 3 separate experiments. *P = 0.025.