Fig. 1.

Quantification of canonical transient receptor potential (TRPC) channels expression in tibialis anterior (TA), extensor digitorum longus (EDL), and soleus (Sol) muscles from TRPC1^+/+ and TRPC1^−/− mice. A: TRPC1^+/+ mice. Each cDNA was amplified in duplicate, and threshold cycle (C_t) values were averaged for each duplicate. The average C_t value for cyclophilin D was subtracted from the average C_t value for the gene of interest, giving ΔC_t (n = 5 or 6). Note that gene expression is inversely proportional to ΔC_t. B: TRPC1^−/− mice. ΔC_t values were obtained from TRPC1^−/− samples (n = 4–6). ND, not detected.