Table 1.
Comparison of organic acids in leaves and roots between control and YK1 rice
| Concentration (nmol g−1 f. wt) |
|||
|---|---|---|---|
| Metabolites | Control (%) | L-1 (%) | L-2 (%) |
| Leaf | |||
| FBP | 21·1 ± 1·0 (100) | 20·1 ± 1·1 (95) | 24·3 ± 4·8 (116) |
| GA3P | 98·5 ± 0·9 (100) | 92·7 ± 6·7 (94) | 82·5 ± 4·0 (84) |
| 3PGA | 1222·3 ± 72·2 (100) | 1101·2 ± 16·6 (90) | 1165·7 ± 95·5 (95) |
| Citrate | 855·5 ± 118·8 (100) | 799·6 ± 69·6 (93) | 762·8 ± 143·4 (89) |
| cis-Aconitate | 18·3 ± 2·3 (100) | 27·2 ± 1·7 (149) | 27·8 ± 0·5 (152) |
| Isocitrate | 101·4 ± 8·8 (100) | 147·1 ± 14·4 (145) | 141·2 ± 22·9 (139) |
| 2-Oxoglutarate | 100·5 ± 4·4 (100) | 127·5 ± 10·0 (127) | 134·0 ± 17·3 (133) |
| Root | |||
| FBP | 143·2 ± 19·4 (100) | 91·7 ± 10·1 (64) | 59·9 ± 24·4 (42) |
| GA3P | 340·2 ± 47·0 (100) | 171·2 ± 9·7 (50) | 138·7 ± 13·0 (41) |
| 3PGA | ND – | ND – | ND – |
| Citrate | 163·1 ± 30·4 (100) | 218·4 ± 70·7 (134) | 226·1 ± 64·3 (139) |
| cis-Aconitate | 2·9 ± 0·2 (100) | 2·3 ± 0·4 (82) | 2·3 ± 0·5 (81) |
| Isocitrate | 14·1 ± 0·5 (100) | 10·2 ± 1·7 (72) | 8·9 ± 0·9 (63) |
| 2-Oxoglutarate | 41·1 ± 4·0 (100) | 39·7 ± 3·3 (96) | 40·4 ± 6·5 (98) |
Leaves and roots of 7-day-old plants were used.
Control, transgenic rice possessing vector alone; L-1 and L-2, transgenic rice possessing the YK1 gene.
Data are the means ± s.e. of three independent experiments.