Table 2.
Comparison of the amounts of free amino acids in leaves and roots
| Concentration (nmol g−1 f. wt) |
|||
|---|---|---|---|
| Metabolites | Control (%) | L-1 (%) | L-2 (%) |
| Leaf | |||
| Arginine | 67·5 ± 4·9 (100) | 67·2 ± 6·9 (100) | 69·9 ± 7·8 (104) |
| Asparagine | 3050·6 ± 29·2 (100) | 2723·2 ± 380·3 (89) | 2997·2 ± 251·6 (98) |
| Aspartate | 1601·2 ± 64·4 (100) | 1695·5 ± 91·1 (106) | 1739·5 ± 72·4 (109) |
| Glutamine | 5050·9 ± 1414·4 (100) | 5233·5 ± 788·4 (104) | 5368·3 ± 706·7 (106) |
| Glutamate | 2965·8 ± 153·1 (100) | 3179·6 ± 36·4 (107) | 3093·0 ± 202·0 (104) |
| Threonine | 287·1 ± 4·9 (100) | 335·8 ± 4·9 (117) | 345·4 ± 22·3 (120) |
| Tryptophan | 24·1 ± 3·6 (100) | 24·4 ± 5·1 (101) | 24·6 ± 2·4 (102) |
| Total | 16 035·6 ± 907·1 (100) | 16 238·3 ± 902·1 (101) | 17 058·2 ± 792·4 (106) |
| Root | |||
| Arginine | 198·3 ± 60·7 (100) | 132·0 ± 6·2 (67) | 111·2 ± 13·7 (56) |
| Asparagine | 1025·2 ± 106·8 (100) | 1068·5 ± 239·0 (104) | 1137·0 ± 159·9 (111) |
| Aspartate | 452·5 ± 31·0 (100) | 468·4 ± 193·0 (104) | 507·7 ± 81·0 (112) |
| Glutamine | 3035·6 ± 296·6 (100) | 2765·5 ± 518·8 (91) | 3052·5 ± 381·5 (101) |
| Glutamate | 690·7 ± 47·3 (100) | 705·6 ± 245·1 (102) | 851·6 ± 97·8 (123) |
| Threonine | 120·9 ± 14·3 (100) | 129·3 ± 27·6 (107) | 121·4 ± 7·6 (100) |
| Tryptophan | 31·9 ± 5·1 (100) | 36·0 ± 5·4 (113) | 42·3 ± 2·8 (133) |
| Total | 8121·0 ± 501·1 (100) | 7850·7 ± 1002·0 (97) | 8041·3 ± 893·1 (99) |
Leaves and roots of 7-day-old plants were used.
Control, transgenic rice possessing vector alone; L-1 and L-2, transgenic rice possessing the YK1 gene.
Data are the means ± s.e. of three independent experiments.