Table 3.
Compositions of free amino acids in seeds of control and YK1 plants
| Concentration (nmol/5 seeds) |
|||
|---|---|---|---|
| Metabolites | Control (%) | L-1 (%) | L-2 (%) |
| Alanine | 19·1 ± 1·2 (100) | 19·1 ± 0·9 (100) | 17·3 ± 1·6 (91) |
| Arginine | 14·7 ± 1·2 (100) | 11·6 ± 0·5 (79) | 13·2 ± 0·9 (91) |
| Asparagine | 107·5 ± 4·0 (100) | 103·7 ± 6·3 (97) | 69·1 ± 3·0 (64) |
| Aspartate | 64·1 ± 4·2 (100) | 63·8 ± 5·9 (100) | 66·2 ± 2·2 (103) |
| Glutamine | 3·9 ± 0·2 (100) | 3·3 ± 0·2 (85) | 2·9 ± 0·4 (74) |
| Glutamate | 118·2 ± 3·7 (100) | 119·1 ± 5·3 (101) | 110·7 ± 6·3 (94) |
| Glycine | 6·4 ± 0·2 (100) | 5·8 ± 0·0 (92) | 6·3 ± 0·4 (100) |
| Histidine | 4·5 ± 0·1 (100) | 3·6 ± 0·1 (81) | 4·9 ± 0·1 (110) |
| Lysine | 2·8 ± 0·1 (100) | 2·6 ± 0·0 (91) | 2·7 ± 0·2 (95) |
| Leucine | 1·7 ± 0·0 (100) | 1·5 ± 0·1 (89) | 1·5 ± 0·0 (87) |
| Methionine | 3·6 ± 0·3 (100) | 3·6 ± 0·1 (102) | 3·0 ± 0·1 (83) |
| Phenylalanine | 1·1 ± 0·1 (100) | 0·9 ± 0·1 (81) | 1·2 ± 0·1 (108) |
| Proline | 18·8 ± 0·3 (100) | 13·9 ± 0·2 (74) | 23·7 ± 1·0 (126) |
| Serine | 20·6 ± 0·5 (100) | 18·4 ± 0·9 (89) | 16·9 ± 0·8 (82) |
| Threonine | 5·0 ± 0·1 (100) | 4·5 ± 0·3 (89) | 5·6 ± 0·4 (110) |
| Tryptophan | 5·7 ± 0·2 (100) | 4·9 ± 0·2 (85) | 10·7 ± 0·7 (187) |
| Tyrosine | 4·1 ± 0·2 (100) | 3·3 ± 0·1 (81) | 4·2 ± 0·3 (102) |
| Valine | 25·5 ± 1·0 (100) | 24·9 ± 0·6 (98) | 18·8 ± 0·6 (74) |
| Total | 427·0 ± 4·9 (100) | 408·5 ± 14·7 (96) | 378·6 ± 12·7 (89) |
Control, transgenic rice possessing vector alone; L-1 and L-2, transgenic rice possessing the YK1 gene.
Data are the means ± s.e. of three independent experiments.