Table 5.
Comparison of organic acid levels in calli of control and YK1 rice
| Concentration (nmol g−1 f. wt) |
||
|---|---|---|
| Metabolites | Control (%) | L-1 (%) |
| FBP | 18·9 ± 1·7 (100) | 28·0 ± 7·3 (148) |
| GA3P | 25·1 ± 2·7 (100) | 11·0 ± 0·9 (44) |
| PEP | 4·6 ± 0·9 (100) | 8·4 ± 2·9 (183) |
| Pyruvate | 17·2 ± 1·3 (100) | 20·3 ± 4·1 (118) |
| Citrate | 95·5 ± 19·3 (100) | 72·3 ± 18·2 (76) |
| cis-Aconitate | 0·6 ± 0·1 (100) | 0·6 ± 0·1 (99) |
| 2-Oxoglutarate | 11·4 ± 0·7 (100) | 15·2 ± 1·3 (134) |
| Succinate | 249·3 ± 9·4 (100) | 183·3 ± 23·8 (74) |
| Fumarate | 48·5 ± 9·2 (100) | 22·2 ± 1·5 (46) |
| Malate | 236·6 ± 28·0 (100) | 161·9 ± 21·4 (68) |
| G6P + F6P | 177·3 ± 12·4 (100) | 212·4 ± 11·5 (120) |
| 6PG | 7·5 ± 1·7 (100) | 9·5 ± 1·9 (126) |
| R5P | 11·8 ± 1·2 (100) | 22·6 ± 2·5 (192) |
Calli cultured for 5 d were used.
Control, transgenic rice possessing vector alone; L-1, transgenic rice possessing the YK1 gene.
Data are the means ± s.e. of three independent experiments.