Table 6.
Comparison of free amino acid levels in calli
| Concentration (nmol g−1 f. wt) |
||
|---|---|---|
| Metabolites | Control (%) | L-1 (%) |
| Alanine | 3136·0 ± 219·0 (100) | 2504·5 ± 242·2 (80) |
| Arginine | 238·5 ± 6·4 (100) | 268·0 ± 18·7 (113) |
| Asparagine | 432·3 ± 102·5 (100) | 372·0 ± 51·4 (86) |
| Aspartate | 85·5 ± 4·3 (100) | 36·8 ± 2·8 (43) |
| Cysteine | 76·5 ± 7·1 (100) | 107·7 ± 5·8 (141) |
| Glutamine | 603·1 ± 21·7 (100) | 200·2 ± 11·5 (33) |
| Glutamate | 715·2 ± 47·5 (100) | 590·6 ± 19·1 (83) |
| Glycine | 705·8 ± 38·1 (100) | 760·1 ± 32·1 (108) |
| Histidine | 124·2 ± 5·8 (100) | 129·0 ± 4·3 (104) |
| Lysine | 279·7 ± 9·5 (100) | 280·4 ± 19·4 (100) |
| Leucine | 486·8 ± 24·2 (100) | 518·5 ± 27·2 (107) |
| Methionine | 54·9 ± 12·3 (100) | 34·2 ± 6·8 (62) |
| Phenylalanine | 416·0 ± 33·9 (100) | 520·4 ± 10·7 (125) |
| Proline | 178·1 ± 9·7 (100) | 159·9 ± 8·8 (90) |
| Serine | 794·6 ± 45·6 (100) | 411·0 ± 29·3 (52) |
| Threonine | 290·5 ± 13·7 (100) | 234·7 ± 20·4 (81) |
| Tryptophan | 54·5 ± 3·1 (100) | 47·4 ± 0·7 (87) |
| Tyrosine | 198·9 ± 9·6 (100) | 193·0 ± 8·2 (97) |
| Valine | 484·8 ± 24·4 (100) | 467·9 ± 34·4 (97) |
| Total | 9355·9 ± 559·3 (100) | 7837·2 ± 218·3 (84) |
Calli cultured for 5 d were used.
Control, transgenic rice possessing vector alone; L-1, transgenic rice possessing the YK1 gene.
Data are the means ± s.e. of three independent experiments.