Table 7.
Comparison of reduced and oxidized glutathione levels in calli
| Concentration (nmol g−1 f. wt) |
|||
|---|---|---|---|
| Metabolites | Control (%) | L-1 (%) | L-2 (%) |
| GSH | 219·7 ± 36·5 (100) | 639·3 ± 105·8 (291) | 449·0 ± 80·4 (204) |
| GSSG | 75·8 ± 11·8 (100) | 99·5 ± 11·3 (131) | 101·5 ± 14·2 (134) |
| GSH + GSSG | 295·5 ± 24·8 (100) | 738·8 ± 117·0 (250) | 550·5 ± 85·3 (186) |
| GSH/GSSG | 2·9 (100) | 6·4 (221) | 4·4 (153) |
Calli cultured for 5 d were used.
Control, transgenic rice possessing vector alone; L-1 and L-2, transgenic rice possessing the YK1 gene.
Data are the means ± s.e. of three independent experiments.