Table 2.
Means ± SE in vitro analyses of muscle biopsies of vastus lateralis muscle
| Wtinitial | Wt−10% | Wtinitial | Wt+10% | |
|---|---|---|---|---|
| Phosphofructokinase, PFK, μM·min−1·g−1 | 56.3±2.9 | 48.7±3.5* | 66.2±5.0 | 73.8±3.2 |
| Creatine kinase, μM·min−1·gm−1 | 407.6±22.1 | 419.6±19.4 | 385.4±22.7 | 413.1±10.3 |
| GAPDH, μM·min−1·g−1 | 441.1±20.2 | 436.7±23.8 | 450.7±30.2 | 496.8±23.2 |
| Citrate synthase, CS, μM·min−1·g−1 | 9.1±0.8 | 9.9 (0.5) | 10.8±0.6 | 11.3±0.9 |
| 2-hydroxyacyl CoA dehydrogenase, HADH, μM·min−1·g−1 | 13.1±0.7 | 13.8±0.5 | 13.9±1.7 | 14.2±3.1 |
| Cytochrome c oxidase, COX; μM·min−1·gm−1 | 5.7±0.5 | 5.8±0.4 | 6.4±0.8 | 7.1±1.0 |
| Hexokinase, μM·min−1·g−1 | 2.4±0.2 | 2.5±0.4 | 2.7±0.2 | 2.9±0.1 |
| Glycogen phosphorylase, μM·min−1·gm−1 | 26.7±1.6 | 28.7±1.9 | 21.6±3.5 | 23.1±3.5 |
| Carnitine palmitoyl tranferase 1, CPT, 1, nM·min−1·g−1 | 0.094±0.005 | 0.089±0.006 | 0.090±0.018 | 0.101±0.004 |
| COX/CS | 0.59±0.02 | 0.59±0.03 | 0.59±0.05 | 0.62±0.04 |
| HADH/CS | 1.70±0.09 | 1.44±0.06 | 1.29±0.05 | 1.25±0.05 |
| PFK/COX | 12.18±1.53 | 9.21±0.70* | 11.0±1.5 | 11.2±1.5 |
Weight loss and weight gain effects on skeletal muscle enzyme activity. Thirty subjects (18 obese, 7 never-obese; 12 males, 13 females) were studied at Wtinitial and Wt−10%. Five subjects (4 never-obese males; 1 never-obese female) were studied at Wtinitial and Wt+10%.
*
P < 0.05 compared to Wtinitial.