Analysis of the Ednra gene recombination in Ednraflox/flox; Dlx5/6-Cre embryos. Genomic PCR analysis of DNA extracted from adult mouse tails (lanes 1–3), embryonic mandibular pharyngeal arches (lanes 4–6) or embryonic yolk sac (lane 7). The genotype of each animal is listed above the lane number. a CrePCR amplification generates a band ~500 bp. b loxP PCR reveals a 375 bp band, representing the wild type (WT) allele, a 425 bp band, representing the mutant allele (flox), or both. c Recombinant PCR detects the recombined Ednraflox allele and generates a 1200 bp band. d Recombinant PCR of DNA extracted from the skin, muscle and bone of the lower jaw of adult Ednraflox/flox; Dlx5/6-Cre mice