Table 4.
The PCR primers for PCR and size of PCR products
| Primer | Target | DNA sequence (5' to 3') | Product Sizesize | Note |
|---|---|---|---|---|
| Tem-F | blaTEM | GAAGATCAGTTGGGTGCACGAGT | 550 bp | This study |
| Tem-R | CAACTTTATCCGCCTCCATCCAGT | |||
| STR-F1 | aadA2 | AGACGCTCCGCGCTATAGAAGT | 203 bp | (46) |
| STR-R1 | CGGACCTACCAAGGCAACGCT | |||
| CS-F | CS region | GGCATCCAAGCAGCAAG | Variable | (47) |
| CS-R | AAGCAGACTTGACCTGA | |||
| 1.9CS-F | Flanking region of CS region | CTGCTGCGTAACATCGTTGCT | Variable | This study |
| 1.9CS-R | GGCGAGATCATCAAGTCAGT | |||
| ColE1-F | ColE1 oriT | CAAATGCTGTCCTTCCAGTGT | 225 bp | This study |
| ColE1-R | CTCAGTTCGGTGTAGGTCGT | |||
| F-F | IncFI oriT | CAACAACGCGCCGACACCGT | 288 bp | This study |
| F-R | CCCTTCCTGTCGACGCTTCT | |||
| R100-F | IncF2 oriT | CCACCAAAAGCACCACACACT | 266 bp | This study |
| R100-R | AGACACTCCTAGCAGCGCCT | |||
| pSC138-F | IncI oriT | TGTCACGAACATCTGCCAGT | 193 bp | This study |
| pSC138-R | GAGAGAAAGTGCCCATGGCT | |||
| IS26in-F | IS26 | GGCACTGTTGCAAAGTTAGC | 820 bp | DQ390455.1 |
| IS26in-R | GGCACTGTTGCAAATAGTCG | |||
| IS26out-F | Variable | GCTAACTTTGCAACAGTGCC | Variable | DQ390455.1 |
| IS26out-R | CGACTATTTGCAACAGTGCC | |||
| Tn-F | Tn | ACCTAGATTCTACGTCAGTAC | Variable | (35) |
| AmpC-F | AmpC | CAAGTTTGATTCCTTGGACTCT | AY253913 | |
| AmpC-R | CTCATCGTCAGTTATTGCAGCT | |||
| SugE-R | sugE | GCCTGATATGTCCTGGATCGT |