. 2009 Dec 28;9:15. doi: 10.1186/1471-2210-9-15

Table 5.

Effects of rapacuronium on ACh-stimulated [³⁵S]GTPγS binding

	Acetylcholine		+ 0.1 μM rapacuronium		+ 1 μM rapacuronium		+ 10 μM rapacuronium
	pEC₅₀	E_MAX	pEC₅₀	E_MAX	pEC₅₀	E_MAX	pEC₅₀	E_MAX
M₁	5.20 ± 0.05	2.23 ± 0.08	5.41 ± 0.04*	2.35 ± 0.12	5.78 ± 0.04*	2.28 ± 0.12	5.22 ± 0.05	2.27 ± 0.11
M₂	6.61 ± 0.03	3.12 ± 0.12	6.65 ± 0.03	3.10 ± 0.15	5.92 ± 0.05*	2.93 ± 0.13	5.67 ± 0.07*	2.46 ± 0.18*
M₃	5.31 ± 0.05	2.12 ± 0.08	5.65 ± 0.04*	2.78 ± 0.11*	5.66 ± 0.04*	2.52 ± 0.08*	4.83 ± 0.05*	2.12 ± 0.09
M₄	5.95 ± 0.04	2.93 ± 0.15	5.93 ± 0.04	2.95 ± 0.11	5.59 ± 0.05*	2.80 ± 0.12	5.12 ± 0.05*	2.32 ± 0.16*
M₅	5.26 ± 0.05	1.99 ± 0.08	5.45 ± 0.04*	2.10 ± 0.09	5.82 ± 0.04*	2.04 ± 0.09	5.29 ± 0.05	2.22 ± 0.09

Negative logarithms of half effective concentrations (pEC₅₀) and maximum stimulatory effect (E_MAX) of acetylcholine on [³⁵S]GTPγS binding in the absence or presence of the indicated concentrations of rapacuronium were obtained by fitting Eq. 5 to the data in Figure 6. Values are means ± SE of fits to 3 independent experiments performed in quadruplicates. *P < 0.05; significantly different from control (Ach alone) by ANOVA and Tukey-Kramer post-test.