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. 2009 Dec 28;187(7):1117–1132. doi: 10.1083/jcb.200909183

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© 2009 Lechtreck et al.

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Figure 7. — Biochemical defects in bbs4-1 flagella. (A) Silver-stained 5–15% SDS–polyacrylamide gel of flagella (FLA), axonemes (AXO), AP, and DP from wild type (g1) and bbs4-1. Proteins present in the AP and DP of bbs4-1 (arrows 1–4) but not in the corresponding fractions from wild type were identified by MS analysis as PLDc (band 1), THB1 (band 2), STPK (band 3), and JGI protein ID 191821 (band 4; see Fig. S3). (B) Details of 2D gels (from Fig. S5 C) separating matrix proteins from wild type (a and c) and bbs4-1 flagella (b and d). Protein spots 1 (b) and 3 (d) were enriched in the matrix of bbs4-1 and revealed to be THB1 and STPK, respectively, by MS. Protein spot 2 (a), which was reduced in quantity in the bbs4-1 matrix, was identified as OEE3. (C) Detail of a silver-stained SDS gel of the DP from the strains indicated. The arrow indicates the position of PLDc. (D) Detail of a silver-stained SDS gel and Western blots of the AP of g1, bbs4-1, and the retrograde IFT mutant dhc1b^ts probed with antibodies to the indicated proteins. The arrow indicates the position of THB1. (A–D) The positions of standard proteins and their molecular masses in kilodaltons are indicated.