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. 2003 Nov 15;17(22):2798–2811. doi: 10.1101/gad.1136103

Table 1.

Ce-dab-1 RNAi phenotypes

RNAia %eglb Laying rate (eggs/h)c Staged % mlte Animals scoredf
Control 1.5 ± 1.4 4.8 ± 1.2 ∼gastrulation 0 720
Ce-dab-1
   1—546g 74.9 ± 9.7 1.8 ± 0.9 ∼pretzel 26.1 ± 6.3 249
   1—293 77.7 ± 5.7 ndh ∼pretzel 25.6 ± 14.6 257
   290—546 75.9 ± 5.2 nd ∼pretzel 31.8 ± 10.2 161
a

L1 animals were soaked overnight in solutions of dsRNA and scored as young adults. Control RNAi was prepared from an empty vector

b

Egg-laying defective animals were scored as young adults by using DIC microscopy and looking for postgastrulation embryos

c

The number of eggs laid by individual animals was counted over several hours and averaged (n = 10)

d

The approximate stage of eggs upon laying were marked as either ∼gastrulation stage (30 to 150 cells) or ∼pretzel stage (400 to 550 cells)

e

Molting-deficient animals were scored by DIC as predominantly young adults. Only animals with body constrictions due to unshed cuticle were scored as positive

f

Numbers represent a total of six independent experiments

g

Numbers represent residues in the corresponding protein sequence

h

nd indicates not determined