Table 1.
Ce-dab-1 RNAi phenotypes
RNAia | %eglb | Laying rate (eggs/h)c | Staged | % mlte | Animals scoredf |
---|---|---|---|---|---|
Control | 1.5 ± 1.4 | 4.8 ± 1.2 | ∼gastrulation | 0 | 720 |
Ce-dab-1 | |||||
1—546g | 74.9 ± 9.7 | 1.8 ± 0.9 | ∼pretzel | 26.1 ± 6.3 | 249 |
1—293 | 77.7 ± 5.7 | ndh | ∼pretzel | 25.6 ± 14.6 | 257 |
290—546 | 75.9 ± 5.2 | nd | ∼pretzel | 31.8 ± 10.2 | 161 |
L1 animals were soaked overnight in solutions of dsRNA and scored as young adults. Control RNAi was prepared from an empty vector
Egg-laying defective animals were scored as young adults by using DIC microscopy and looking for postgastrulation embryos
The number of eggs laid by individual animals was counted over several hours and averaged (n = 10)
The approximate stage of eggs upon laying were marked as either ∼gastrulation stage (30 to 150 cells) or ∼pretzel stage (400 to 550 cells)
Molting-deficient animals were scored by DIC as predominantly young adults. Only animals with body constrictions due to unshed cuticle were scored as positive
Numbers represent a total of six independent experiments
Numbers represent residues in the corresponding protein sequence
nd indicates not determined