Table 2.
Adaptin complexes and EGL-17 secretion
| Genea | Adaptin complexb | Subunitb | Functionb | EGL-17::GFP accumulationc | mltd |
|---|---|---|---|---|---|
| apm-1 | AP-1 | μ1/μ1B | secretion | + | — |
| apt-10 | + | — | |||
| unc-101 | + | — | |||
| apt-2 | + | — | |||
| apt-4 | AP-2 | α | endocytosis | n/ae | n/ae |
| apt-5 | AP-3 | δ | golgi-lysosome traffic | — | — |
| apt-6 | β3 | — | — |
a
L1 animals were soaked overnight in solutions of dsRNA and scored as L3 larvae. Control RNAi was prepared from an empty vector
b
Presumptive adaptin complex, subunit, and function by homology with mammalian proteins
c
Accumulation of the EGL-17::GFP fusion protein was measured in the descendants of P6.p as described
d
Molting-deficient animals were scored by DIC as L3/L4 larvae
e
RNAi was lethal prior to larvae reaching L3/L4 stage