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. 2009 Dec 28;187(7):1055–1069. doi: 10.1083/jcb.200908040

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© 2009 von Blume et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 8. — A subset of plasma membrane proteins are missorted upon ADF and cofilin1 knockdown. ADF and cofilin1 (CFL-1) knockdown affects the polypeptide composition of plasma membrane. HeLa cells were transfected with control or ADF + cofilin1 siRNA. The cells were pulsed with ³⁵S-labeled methionine for 15 min and chased with unlabeled L-methionine for 2 h with or without BFA. Subsequently, intact cells were surface biotinylated with NHS-biotin and lysed. Equal amounts of lysates (based on [³⁵S]methionine content) from control and ADF/cofilin1 knockdown cells were incubated with avidin-conjugated beads. The plasma membrane population of polypeptides in ADF + cofilin1 knockdown cells was different compared with control cells. N, new proteins; L, less or missing; *, no obvious change.