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. 2009 Dec 28;187(7):1055–1069. doi: 10.1083/jcb.200908040

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© 2009 von Blume et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 9. — ADF and cofilin1 knockdown causes swelling of the TGN. (A) HeLa cells were transfected with control or ADF + cofilin1 (CFL-1) siRNA. The cells were fixed and processed for electron microscopy. (B) HeLa treated with control or ADF + cofilin1 siRNA were transfected with HRP-TGN38, and the organization of Golgi membranes and the localization of TGN38 were monitored by electron microscopy as described previously (Bossard et al., 2007). Bars, 180 nm.