Table I.
Secretion of cargoes unchanged upon ADF and cofilin1 knockdown (class A)
| Protein name | Unique peptides | Ratio (H/L) control/RNAi |
| Matrix-remodeling–associated protein 5 | 14 | 1 |
| Fibulin 1 | 1 | 1 |
| Procollagen C-endopeptidase enhancer 1 | 5 | 1 |
| Carboxypeptidase A4 | 47 | 1 |
| Follistatin-related protein 1 | 35 | 1 |
| Laminin subunit α5 | 30 | 0.9 |
| Fibrillin-1 | 4 | 0.9 |
| Fibronectin | 12 | 0.8 |
Secretion of proteins into cell culture media from control and ADF and cofilin1 siRNA–treated HeLa cells was analyzed by SILAC-based MS. There was no change in secretion of cargoes classified as class A upon ADF and cofilin1 knockdown (ratio between heavy and light peptides [H/L] = 1).