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. 2009 Dec 28;187(7):1071–1082. doi: 10.1083/jcb.200908075

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© 2009 Ally et al.

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Figure 4. — Ncd restores kinesin-1–driven transport. (A) Schematic of Pex3-mCherry–Ncd(700) constructs. Drosophila Pex3 is fused to mCherry and human Ncd encoded by amino acids 201–700. (top) Pex3-mCherry–Ncd(700) with wild-type neck linker. (bottom) Motility-deficient Pex3-mCherry–Ncd(700)^ran12 with mutated neck linker (Sablin et al.,1998). (B) Pex3-mCherry–Ncd(700) expression results in accumulation of peroxisomes at the tips of processes. S2 cells stably transfected and plated in the presence of CytoD in an endogenous DHC-depleted background. DIC, differential interference contrast. Arrowheads highlight peroxisomes in processes. Bars, 5 µm. (C) Quantitative representation of results showing changes in the distribution of peroxisomes along processes before and after DHC depletion and expression of Pex3-mCherry–Ncd. Data represent mean values ± SD from 120 cells per condition from three separate dsRNA treatments. Asterisks indicate statistical significance using the Student’s t test.