Table 3.
ADP-kinetics of respiration in permeabilized fibres in fish R-solution
| Temp | Creatine | n | V0 | Vmax | ACR | KM ADP |
|---|---|---|---|---|---|---|
| 10°C | - | 6 | 2.34 ± 0.21 | 24.85 ± 3.44 | 10.68 ± 1.22 | 783 ± 160 |
| + | 6 | 2.01 ± 0.19 | 22.6 ± 3.83 | 11.03 ± 1.62 | 376 ± 55 * | |
| 15°C | - | 5 | 2.78 ± 0.32 | 29.13 ± 3.16 | 10.66 ± 1.01 | 579 ± 64 |
| + | 5 | 2.51 ± 0.08 | 27.25 ± 2.22 | 10.96 ± 1.11 | 301 ± 42 * | |
| 20°C | - | 5 | 3.35 ± 0.07 | 28.06 ± 3.37 | 8.40 ± 1.05 | 293 ± 115 |
| + | 5 | 3.37 ± 0.15 | 30.59 ± 1.83 | 9.10 ± 0.52 | 224 ± 42 |
Notation and statistical tests are as in Table 1. * denotes P < 0.05 significantly different in the presence of creatine. Temperature had a significant effect on V0 (P < 0.05 and P < 0.001 in the absence and presence of creatine, respectively) and KM ADP in the absence of creatine (P < 0.05).