Figure 11.

Effects of PF-228 on the phosphorylation of FAK, Akt and ERK in pancreatic cancer cells. A, Panc-1 cells were treated with or without the indicated concentrations of PF-228 for 1 h. B, After pretreated with or without the indicated concentrations of PF-228 for 30 min in serum-free medium, suspended AsPC-1 cells were plated onto plastic or LN-coated plates for 1 h in the continued presence or absence of PF-228. Western blot showed expression of pFAK (pY397), p-Akt (pS473), p-ERK 1/2 and their total proteins in the cells. The membranes were probed with anti-β-actin antibody to ensure even loading of proteins in each lane.