Figure 14.

Effects of PF-228 on the expression of apoptosis-associated proteins in pancreatic cancer cells. A, Panc-1 cells were treated with or without the indicated concentrations of PF-228 for 24 h. B, After pretreated with or without the indicated concentrations of PF-228 for 30 min in serum-free medium, AsPC-1 cells were plated onto plastic or LN for 24 h in the continued presence or absence of PF-228. Western blot analysis was used to detect the expression of Bad, p-Bad (pS136), p-Bad (pS112), Bcl-2, Bax and survivin. The membranes were probed with anti-β-actin antibody to ensure even loading of proteins in each lane.