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. 2009 Dec 21;8:125. doi: 10.1186/1476-4598-8-125

Figure 3.

Figure 3

Effects of FAK RNAi and FRNK overexpression on the expression and phosphorylation of FAK in Panc-1 cells. Western blot analysis showed expression of FRNK, total FAK and pFAK (pY397) in Panc-1 cells transiently transfected with FAK RNAi plasmids (FAK RNAi1 and FAK RNAi2) for five days (A) or pcDNA3.1-FRNK plasmid (FRNK) for three days (B). Parental cells and their respective vector-transfected (RNAi vector, vector) cells served as the control. C, FAK RNAi2 plasmid-transfected (i-Pool 1, i-Clone 3) and vector-transfected (i-Vector) clones derived from Panc-1 cells were obtained using blasticidin as a selection marker. Western blot showed expression of pFAK (pY397), p-Akt (pS473), p-ERK 1/2 and their total proteins in the cells. D, pcDNA3.1-FRNK plasmid-transfected (Pool 1, Clone 2) and empty vector-transfected (Vector) clones were obtained using G418 as a selection marker. Western blot showed expression of pFAK (pY397), p-Akt (pS473), p-ERK 1/2 and their total proteins in the cells. The membranes were probed with anti-β-actin antibody to ensure even loading of proteins in each lane.