Table III.
Summary of structural components present or absent in WT and drc mutants
Structural component | Strain | ||||
WT/pWT | sup-pf-4 | sup-pf-3 | pf2 | pf3 | |
DRC linker (1,000–1,200) | |||||
Nexin link connection to neighboring B-tubule | |||||
Proximal lobe (∼350) | + | + | − | − | − |
Distal lobe (∼120) | + | +/− | − | − | − |
Linker projections | |||||
L1 (50–80) | + | + | +/− | − | +/− |
L2 | + | − | − | − | − |
OID linker | + | + | +/− | − | +/− |
Central linker region | |||||
Central linker | + | + | +/− | − | +/− |
Docking subunit (∼60) | + | + | + | + | + |
DRC base plate (300–350) | |||||
Base plate protrusion (∼80) | + | + | + | − | − |
B11-A3 section | + | + | + | + | − |
Connection to B11 | + | + | + | + | − |
B-tubule hole | + | + | + | + | − |
IDAs | |||||
IA4 | + | + | +/− | +/− | − |
IA5 | + | + | + | +/− | (−) |
+ indicates that the component is present; +/− indicates that the component reduced; (−) indicates that the component is almost completely reduced; − indicates that the component is lacking. Molecular mass is indicated in parentheses (in kilodaltons).