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. 2009 Dec 14;187(6):905–920. doi: 10.1083/jcb.200908019

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© 2009 Pearson et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 7. — Zebrafish Dr.poc1B morphants exhibit ciliopathy defects. (A) Dr.poc1B morphants (DrPoc1B MO) display smaller eyes, heart edemas (red arrow), mislocalized melanocytes (green arrow), truncated bodies, and kidney cysts (white arrow) at 4 d post fertilization (dpf). Bars, 0.5 mm. (B) Cilia (green) of Dr.poc1B morphant embryos at 48 hpf are disorganized and appear shorter within the pronephretic duct (PND) and are expanded within the posterior neural tube (NT). Short cilia were visible in the KV at 12 hpf (Dr.poc1BATG MO, 3.6 ± 0.9 µm; Dr.poc1BSPL MO, 3.6 ± 0.9 µm; control, 6.2 ± 0.8 µm; n > 100; P < 0.001). Bars: (top) 10 µm; (middle) 25 µm; (bottom) 5 µm. (C) Heart laterality defects were observed in Dr.poc1B morphants at 48 hpf. Using in situ hybridization to cmlc2, heart loops were visualized in flat-mounted sections. The ratios indicate the relative frequency of heart positioning. Bar, 0.5 mm. (D) Fish were stained with Alcian blue to visualize cartilage and bone. Morphants displayed craniofacial abnormalities consistent with the ciliopathy phenotype. Dr.poc1B morphants exhibit pharyngeal arch and jaw defects at 6 d post fertilization. PQ, palatoquadrate; M, Meckel's cartilage; CH, ceratohyal; VPA, visceral pharyngeal arches. Bar, 0.5 mm.