Table 3.
The effect of repeat unit sequence on microsatellite heterozygosity for tetra-nucleotide loci
| AAAT & TTTA | AATG | CATA & GTAT | TTCA | GATG | ATCT & TAGA | AAGG & TTCC | |
|---|---|---|---|---|---|---|---|
| Number of loci | 30 | 4 | 8 | 3 | 5 | 253 | 18 |
| Mean He | 0.683 | 0.691 | 0.714 | 0.719 | 0.722 | 0.744 | 0.789 |
| AAAT [AATA-ATAA-TAAA] & TTTA [TTAT-TATT-ATTT] | 0.979 | 0.407 | 0.416 | 0.421 | 4.49 × 10-4 | 4.80 × 10-5 | |
| AATG [ATGA-TGAA-GAAT] | 0.808 | 0.857 | 0.556 | 0.229 | 0.066 | ||
| CATA [ATAC-TACA-ACAT] & GTAT [TATG-ATGT-TGTA] | 0.921 | 1 | 0.239 | 0.011 | |||
| TTCA [TCAT-CATT-ATTC] | 0.786 | 0.541 | 0.080 | ||||
| GATG [ATGG-TGGA-GGAT] | 0.304 | 0.019 | |||||
| ATCT [TCTA-CTAT-TATC] & TAGA [AGAT-GATA-ATAG] | 0.003 | ||||||
| AAGG [AGGA-GGAA-GAAG] & TTCC [TCCT-CCTT-CTTC] | |||||||
P values are shown for two-sided Wilcoxon rank-sum tests for differences in heterozygosity (He) between the different repeat unit sequences of microsatellites with one tetra-nucleotide STR region. Four loci were excluded from these comparisons because their repeat unit sequences only appeared once (TTTG and GAAA) or twice (TCCA) in the data set. Tests with P < 0.05 are highlighted in bold.