Figure 1.

Region upstream of glmUS, including site of beneficial mutation. NagC binds to two 23-bp BoxG elements shown in bold. The beneficial adenine insertion was in a tract of seven existing adenines in BoxG1; it is shown above the sequence as +A. The Shine-Dalgarno sequence (S-D) and the -10 and -35 regions of two promoters, P1 and P2, are underlined. Figure modified from Plumbridge [31].