Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2009 Dec;134(6):489–521. doi: 10.1085/jgp.200910314

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2009 Moss et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jgp.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 5. — Expression and functional characterization of C-terminal insertion mGAT1XFPCT constructs. (A) Each panel displays exemplar fluorescence microscopy images of live N2a cells expressing each of the C-terminal insertion constructs 48 h after transfection. Note that the subcellular distributions of mGAT1⁵⁶⁵CFP⁵⁶⁶CT and mGAT1⁵⁶⁵YFP⁵⁶⁶CT differ in the displayed panels. These variations are not caused by the different fluorophores but are displayed to exemplify each of the two types of subcellular distributions observed for this construct design. Bars, 10 µm. (B) 20-min uptake of 2.5 µM GABA/25 nM [³H]GABA from N2a cells transfected with 100 ng/well of mGAT1 wild-type plasmid, an equimolar amount of the fluorescently tagged mGAT1 plasmids, or blank pcDNA3.1(+) vector. For these experiments, 100% wild-type [³H]GABA uptake is 5.3 ± 0.6 fmol/µg/min. Results represent the mean ± SEM of 6–12 transfections for each construct. *, significant difference compared with wild-type; P ≤ 0.05 (ANOVA with Tukey's post-hoc test). (C) GABA concentration dependence of mGAT1⁵⁷⁷YFP⁵⁷⁸CT compared with wild-type mGAT1. Each data point represents the mean ± SEM of six transfections of each construct at each [GABA]_O. (D) Left-hand panels display representative expression pattern of XFP15mGAT1 constructs in N2a cells (bar, 10 µm) 48 h after transfection. Right-hand graph displays the results of 20-min [³H]GABA uptake from N2a cells transfected with 100 ng/well of mGAT1 wild-type plasmid, an equimolar amount of the XFP15mGAT1 plasmids, or blank pcDNA3.1(+) vector. 100% wild-type [³H]GABA uptake is 21.4 ± 1.8 fmol/µg/min. Results represent the mean ± SEM of 18 wild-type mGAT transfections and 6 transfections of CFP15mGAT1 and YFP15mGAT1. *, significant difference compared with wild-type; P ≤ 0.05 (ANOVA with Tukey's post-hoc test).