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. 2009 Dec 21;206(13):3015–3029. doi: 10.1084/jem.20090847

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© 2009 Francisco et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jem.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 2. — PD-L1–induced CD4⁺ Foxp3⁺ T reg cells suppress CD4⁺ T eff cells in vitro. (A) PD-L1 iT reg cell function was assessed by [³H]thymidine incorporation of naive CD4⁺CD25⁻ T eff cells after 3 d of co-culture at a 1:1 T reg/T eff cell ratio plus PD-L1 beads (5:1 bead/T eff cell ratio). Data represent the mean ± SD and are representative of at least two independent experiments. (B) PD-L1 iT reg cell function was assessed by CFSE dilution of naive CD4⁺CD25⁻ T eff cells after 3 d of incubation with 1:1 T reg/T eff cell ratio and PD-L1 beads (5:1 bead/T eff cell ratio). Data represent the mean ± SD and are representative of three similar experiments. (C) Quantification of T eff cell proliferation in B, analyzing the division index of gated CD4⁺CD45.1⁺ (the number of divisions a single cell has divided) by FlowJo software. Data represent the mean ± SD.