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. 2009 Dec 21;206(13):3015–3029. doi: 10.1084/jem.20090847

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© 2009 Francisco et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jem.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 3. — PD-L1 maintains Foxp3 expression by iT reg cells during suppression of effector cell function. (A) Schematic depiction of experiment. Naive CD4⁺CD62L^hiFoxp3.GFP⁻ T cells were induced toward T reg cell differentiation for 3 d in the presence of TGF-β plus IL-2 and either control or PD-L1 beads. Foxp3.GFP⁺CD45.1⁻ cells were then sorted and co-cultured with sorted CD4⁺CD25⁻CD45.1⁺ in the presence of either control or PD-L1 beads during the 3-d suppression assay. (B) 72 h after co-culture, CD4⁺CD45.1⁻ cells were gated and analyzed for GFP expression. (C) Quantification of experiment depicted in B. Data represent the mean ± SD and are representative of at least two independent experiments.